A rapid and mini-scale protocol of DNA extraction for PCR from Aspergillus section Flavi and other food-borne filamentous fungi
نویسندگان
چکیده
منابع مشابه
A simple and rapid DNA extraction protocol for filamentous fungi efficient for molecular studies
A simple and rapid protocol for extracting high-quality DNA from filamentous fungi was studied. The method involved disruption of fungal cells by employing glass bead method, followed by inactivation of proteins using CTAB/proteinase K. The DNA yield from fungal isolates varied from 310-1879 μg g dry mycelium and a clear intact DNA band was observed upon agarose gel electrophoresis. Absorbency ...
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Background: Contamination of stored pistachio nuts to Aspergillus section Flavi and aflatoxins (AFs) as well as effective parameters on fungal growth and AF production during storage was assayed. Methods: Twenty one pistachio nut samples were taken from some warehouse of Damghan county. Different parameters of storage, including temperature, relative humidity, and pest infestation were recorded...
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ژورنال
عنوان ژورنال: Mycotoxins
سال: 2004
ISSN: 0285-1466,1881-0128
DOI: 10.2520/myco.54.39